Journal: PLoS ONE

Article Title: Morphological association between the muscles and bones in the craniofacial region

doi: 10.1371/journal.pone.0227301

Figure Lengend Snippet: Panels A, B, E, F, I, J show the frontal sections at E12.5. Panels C and D (G and H, K and L) show high magnification view of panels A and B (E and F, I and J). Yellow dots indicate anlage of the TVP (panels C, D, G, H, K, and L). Panels A, B, E, F, I, J (C, D, G, H, K, and L) show same magnification level. The TVP first appears medial to the medial pterygoid (M) at E12.5 (panels A, B, E, F, I, and J). The desmin-positive TVP (FITC) is located lateral to the Sox9-positive inferior spine (S) (Rhodamine) (panels A, B, E, F, I, J). The anterior TVP in the C57BL6J mice had a larger CSA than that in the BALB/cA mice ( P < 0.05) (panel M). There were no differences in the primordial muscle size between the C57BL6J and BALB/cA mice in the middle ( P = 0.79) or posterior ( P = 0.24) parts of the TVP (panels N and O). L. lateral pterygoid; M. medial pterygoid; MA. masseter; S. inferior spine of the hypophyseal cartilage; T. tensor veli palatini; Scale bar = 100 μm.

Article Snippet: Sections were incubated overnight at 4°C with the following primary antibodies: mouse anti-desmin antibody (1:1000, Merck Millipore) and rabbit anti-sox9 antibody (1:1000, Merck Millipore).

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: Isolation of human foetal myoblasts and its application for microencapsulation

doi: 10.1111/j.1582-4934.2007.00119.x

Figure Lengend Snippet: Myogenic enrichment examined by desmin staining. ( A ) Fibroblasts at passage 2 under phase contrast microscopy; ( B ) The same fibroblasts at passage 2 under light transmission microscopy; ( C ) C2C12 cell line; ( D ) Human primary myoblasts at passage 5. All the cells were grown for 24 hrs, fixed with 95% methanol and stained with anti-desmin antibody. (Magnification: × 160)

Article Snippet: Cells were fixed in 95% methanol at –20°C for 20 min., rinsed in PBS and incubated in blocking solution (1% BSA + 0.5% Triton-100 in PBS) for 30 min. A monoclonal antibody of mouse anti-human desmin (PharMingen, Becton Dickinson Inc., San Jose, CA, USA) was applied at a dilution of 1:1000 in blocking solution for 1 hr at room temperature.

Techniques: Staining, Microscopy, Transmission Assay